Fig. 4: Single-trigger-site CAST operations triggered by thrombin. | Nature Communications

Fig. 4: Single-trigger-site CAST operations triggered by thrombin.

From: Programming conformational cooperativity to regulate allosteric protein-oligonucleotide signal transduction

Fig. 4

a, b Schematic and design illustration of single-trigger-site CAST triggered by thrombin. c PAGE gel analysis of single-trigger-site CAST. Lane 1: D; Lane 2: CE-29apt; Lane 3: CE-29apt + Thr; Lane 4: CDE-29apt; Lane 5: CDE-29apt + Thr. [DNA complex] = 0.6 µM, [Thr] = 1.5 µM. d Fluorescence output of single-trigger-site CAST varying thrombin concentrations. d [DNA complex] = 0.6 µM, [Thr] = 0, 0.3 µM, 0.6 µM, 0.9 µM, 1.2 µM, and 1.5 µM. Illustrations (e) and designs (f) of the single-trigger-site CAST triggered by thrombin with different T3 lengths, respectively. g Fluorescence results of the single-trigger-site CAST triggered by thrombin with different T3 lengths of 3, 8, 13, 20 and 30 nt for complex-T3 (1), (2), (3), (4), (5). [complex-T3] = 0.6 μM and [Thr] = 1.5 μM.

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