Fig. 4: Fluorescence intensity and lifetimes of PEG-induced in vitro mCherry precipitates in solution. | Nature Communications

Fig. 4: Fluorescence intensity and lifetimes of PEG-induced in vitro mCherry precipitates in solution.

From: Fluorescent protein lifetimes report densities and phases of nuclear condensates during embryonic stem-cell differentiation

Fig. 4

a–d Fluorescence lifetime (a, c) and intensity (b, d) images of mCherry PEG 6000-induced in vitro precipitates formed after adding mCherry at a total concentration of 100 nM in a solution containing 25% FVO (a, b) and 50% FVO (c, d) PEG 6000. e A few frames from a time series of fluorescence lifetime images of mCherry precipitates formed in a solution containing 50% PEG 6,000, with white arrows pointing on precipitates with visible changes in fluorescence lifetime over the course of 140 s, but with a homogeneity of lifetime values in each time point for each precipitate. Fluorescence lifetime colorbar scale from 0.5 and 1.5 ns.

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