Fig. 5: fluorescence lifetimes of mCherry within mCherry-tagged histone protein 1α (HP1α) condensates are lower and more heterogeneous in undifferentiated than in early-differentiated mouse embryonic stem cells (ESCs). | Nature Communications

Fig. 5: fluorescence lifetimes of mCherry within mCherry-tagged histone protein 1α (HP1α) condensates are lower and more heterogeneous in undifferentiated than in early-differentiated mouse embryonic stem cells (ESCs).

From: Fluorescent protein lifetimes report densities and phases of nuclear condensates during embryonic stem-cell differentiation

Fig. 5

a–f Undifferentiated ESCs. Two examples of undifferentiated ESCs with mCherry-HP1α condensates, fluorescence intensity images (a, d), fluorescence lifetime images (b, e) and the histogram of pixel fluorescence lifetimes (c, f). g–l Early-differentiated ESCs. Two examples of early-differentiated ESCs with mCherry-HP1α condensates, fluorescence intensity images (g, j), fluorescence lifetime images (h, k) and the histogram of pixel fluorescence lifetimes (i, l). A vertical red line shows the 1.44 ns lower boundary of mCherry fluorescence lifetime below which the crowdedness level is above 30% FVO (Fig. 1). Scale bar 2 µm. Fluorescence lifetime colorbar scale from 1.00 and 1.75 ns.

Back to article page