Fig. 4: Mild drought causes PSR and severe drought induces ABA response in pot-grown soybean plants. | Nature Communications

Fig. 4: Mild drought causes PSR and severe drought induces ABA response in pot-grown soybean plants.

From: Phosphate starvation response precedes abscisic acid response under progressive mild drought in plants

Fig. 4

a Time-course analysis of VWCs in potted soybean plants with five different moisture contents (n = 6 independent replicates). The range of variation in VWCs during the period from day 7 after sowing (when plants were transplanted into pots) to day 38 (when sampling was performed) is shown on the right. Drought stress treatments were imposed 10 days after sowing. Relationships between soil VWC and water potential were 36.9% (−0.0039 MPa, pF 1.6), 32.7% (−0.0098 MPa, pF 2.0), 30.0% (−0.031 MPa, pF 2.5), 28.1% (−0.098 MPa, pF 3.0), and 19.1% (−0.61 MPa, pF 3.8). b Thermogram and a corresponding digital image of 26-day-old soybean plants in pots. c, d Relative expression of PSR (c) and ABA-responsive (d) genes in the first trifoliate leaves of the 38-day-old plants shown in b, as determined by RT-qPCR (n = 6 biologically independent replicates). The actin gene (Glyma.15G050200) was used as an internal control for gene expression. *P < 0.05, **P < 0.01, and ***P < 0.001, one-way analysis of variance (ANOVA) with Tukey’s test in WC2–WC5 versus WC1. Error bars denote SD. e Proposed model of the plant’s response to increasing levels of water deficit stress in terms of gene expression.

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