Fig. 3: C16orf72-depleted cells display elevated levels of DNA damage. | Nature Communications

Fig. 3: C16orf72-depleted cells display elevated levels of DNA damage.

From: C16orf72/HAPSTR1/TAPR1 functions with BRCA1/Senataxin to modulate replication-associated R-loops and confer resistance to PARP disruption

Fig. 3

a Quantitative image-based cytometry (QIBC) of γH2AX levels in wild-type U2OS and C16orf72 knock-out cells (C16orf72Δ.2 and C16orf72Δ.3) treated with 2 mM hydroxyurea (HU) for 0, 1 h, 2 h, 8 h or 24 h. Left: representative images of γH2AX induction in the cells. Scale bar represents 20 µm. Right: Mean % γH2AX positive cells ± SEM of 3 biological independent experiments with at least 500 cells analysed per condition. b QIBC co-staining of γH2AX and RPA70 in wild-type U2OS and C16orf72 knock-out cells (c16orf72Δ.2 and c16orf72Δ.3) treated with 2 mM hydroxyurea (HU) for 24 h. Left: representative results of elevated γH2AX and RPA70 levels in c16orf72Δ.2 cells. Right: mean values ± SEM of 3 biological independent experiments. Statistical analysis from at least 3 independent experiments was performed using Ordinary one-way ANOVA with a Bonferroni post-hoc analysis; **p < 0.01; ****p < 0.0001. Source data are provided as a Source Data file.

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