Fig. 3: Immune contexture across Myc;Ptenfl subtypes.
A Example of multiplex immunohistochemistry (mIHC) images from Myc;Ptenfl Stromal-rich (n = 21) and Stromal-poor tumors (n = 13) with Myeloid markers expression on the expression of the left and lymphoid marker on the right. Scale bars = 100 µm. B Total immune cells by CD45+ density in Stromal-rich tumor (n = 21 biologically independent samples) periphery, border, and core compared to Stromal-poor tumors (n = 13 biologically independent samples). Left graph; box-and-whisker plots show median and interquartile range (* represent significant p value < 0.05 using two-way ANOVA (mixed model)), the centerline of the boxplots represents the median value (50th percentile), and the box encapsulates the range from the 25th to the 75th percentiles of the dataset. The whiskers extend from the minimum to the maximum values, showcasing the full spread of the data. Middle image; Tumor border was determined by CD45 and PANCK expression (supplemental S4A and B. Scale bars = 100,000 µm). On the right, unsupervised clustering; heatmap color computed as the z-score of log10 normalized celltype density (cells/mm2) in each periphery, border, and core of Myc;Ptenfl tumor subtypes. C Sankey diagrams showing the distribution of each immune lineage population in stromal-rich (left) and stromal-poor (right) tumors; periphery, border, and core.
