Fig. 1: Identification of Histone H3 Ser-57 phosphorylation (H3S57ph) in vertebrates.
From: Histone H3 serine-57 is a CHK1 substrate whose phosphorylation affects DNA repair
a MS/MS spectrum of a histone H3 peptide bearing phosphorylated Ser-57 noted by the ions y7, y8 and y9 with neutral loss (represented by *); 5 different egg extracts were used. b Western blot analysis (WB) of chromatin purified from interphase Xenopus egg extracts during DNA replication, treated with buffer or lambda phosphatase, amidoblack for loading control; n = 2 independent experiments. c WB (18% PAGE) of acid-extracted histones from a panel of human cell lines; n = 1 experiment. d WB (18% PAGE) of sucrose gradient-separated nucleosome fractions. H3.cs1 recognises the clipped form of H3; n = 2 independent experiments. e WB (18% PAGE) of purified, native mono-nucleosomes after in vitro cleavage of their N-terminal tails by trypsin; n = 3 independent experiments. Source data are provided as Source Data file.
