Fig. 9: Effects of RT, CTLA4i, and anti-CD40 on spontaneous 4T1 lung metastasis and lung immune cells.

BALB/c mice implanted with 4T1 tumors were treated with the schedule in Fig. 6. Lungs were collected at day 22 to evaluate the immune infiltrate and tumor cell content. a Number of clonogenic 4T1 lung metastasis at day 22. Each dot represents a single replicate (4 to 6 replicate cultures per mouse, 10 biologically independent mice per group). Square root transformed data were used to ensure the underlying model assumptions were satisfied. P-values were adjusted for multiple comparisons by controlling the false discovery rate. Horizontal lines indicate the median, Q1 and Q3. b Percentage of CD3⁺ T cells among CD45⁺ cells. c Percentage of CD8⁺ T cells among CD45⁺ cells and their expression of CD69 (d) and PD1 (e). f Percentage of AH1-specific CD8⁺ T cells defined by dextramer staining. (g) Percentage of CD4⁺ T cells among CD45⁺ cells. Expression of CD69 (h) and PD1 (i) by conventional (conv) CD4⁺ T cells, defined as FOXP3^-. j Percentage of CXCR3⁺ among conventional CD4⁺ T cells. k Percentage of DCs, defined as Ly6G^- CD11c⁺ F4/80⁻ among CD11b⁺ cells. Percentage of expression (l) and expression levels (m) of CD40 by DCs. n Percentage of XCR1⁺ DCs. b–n Data are shown as mean ± SD, each dot represents one animal, n = 10 biologically independent mice per group. Populations were compared by Kruskal–Wallis and Dunn’s post-test for statistical significance. *, **, *** and **** indicate p-values < 0.05, 0.01, 0.001 and 0.0001 respectively. Source data and exact p values are provided in the Source Data file.