Fig. 2: Fast and slow migrating cells show distinct intracellular diffusion maps and lamellipodium structures. | Nature Communications

Fig. 2: Fast and slow migrating cells show distinct intracellular diffusion maps and lamellipodium structures.

From: Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion

Fig. 2

a–c QD diffusion maps (a), QD trajectories (b), and CMFDA fluorescence images (c) for migrating cells with fast and slow speeds and for cells treated with 10 nM latrunculin A (LatA) or 50 μM blebbistatin (Bleb). The color range from blue to red corresponds to Dp (local diffusion rate) from 0 to 0.8 μm2/s. The cells are to move upwards, with their outlines and cell body regions labeled by yellow lines. d STED imaging of actin filaments labeled by SiR-actin in fixed cells at the four indicated cell conditions. e Average D and migration speeds for cells at four conditions (fast, n = 16; slow, n = 16; LatA, n = 15; Bleb, n = 14). Data are shown as mean ± SD. f, g Normalized intensity profiles of QD movie projection (f) and CMFDA (g) along a line across the lamellipodium, from the cell body to its leading edge (dotted line). h FRAP of the CMFDA intensity in the front lamellipodium at four conditions, with the fitted recovery times: 0.73 ± 0.18 s (fast, n = 35), 1.09 ± 0.37 s (slow, n = 40), 1.06 ± 0.30 s (LatA, n = 19) and 1.04 ± 0 .25 s (Bleb, n = 20). The solid lines and the shaded regions represent mean ± SE. Inset schematic for the FRAP region in the front lamellipodium. Scale bar, 10 μm. Source data are provided as a Source Data file.

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