Fig. 6: Direct interhemispheric connections to different types of ACA neurons detected by RV-mediated trans-synaptic retrograde tracing.
From: A frontal transcallosal inhibition loop mediates interhemispheric balance in visuospatial processing
a Viral vectors and injection procedure for RV-mediated trans-synaptic retrograde tracing from the indicated types of ACA neurons. b Viral injection sites in the ACA of CaMKIIα-, PV-, SST-, and VIP-Cre mice (scale bar, 1 mm). Inset, enlarged view of the region in the white box showing starter cells for RV tracing (yellow, scale bar, 20 μm). Green, EGFP; red, mCherry; blue, DAPI. Pyr, n = 3 mice; PV, n = 5; SST, n = 3; VIP, n = 5. c Distribution of starter cells in each group (Pyr, n = 3 mice; PV, n = 5; SST, n = 3; VIP, n = 5). d RV-labeled neurons in the contralateral ACA (red box in coronal diagram) of CaMKIIα-, PV-, SST-, and VIP-Cre mice (scale bar, 200 μm). Pyr, n = 3 mice; PV, n = 5; SST, n = 3; VIP, n = 5. e Detected RV-labeled neurons in the contralateral hemisphere in each group. Dots, detected neurons. f Percentages of RV-labeled interhemispheric presynaptic neurons in each region among all the RV-labeled neurons in the contralateral hemisphere (Pyr, n = 3 mice; PV, n = 5; SST, n = 3; VIP, n = 5). Included are areas with >2% labeling. Data are presented as the mean ± SEM. Source data are provided as a Source Data file.
