Fig. 8: ACA PV+cal neurons show an ipsilateral bias in the 2AUC change-detection task.
From: A frontal transcallosal inhibition loop mediates interhemispheric balance in visuospatial processing
a Schematic diagrams of the viral strategy (top) and calcium imaging of PV+cal neurons (bottom). b Raw fluorescence trace of an example PV+cal neuron. Same behavior paradigm as the test phase shown in Fig. 1h, containing the trials at 100% and 12.5% contrast levels. Dark color, high contrast; light color, low contrast. Purple shading, visual white noise; red shadings, contralateral gratings; blue shading, ipsilateral gratings. Red and blue arrowheads, end of wheel rotation in the contralateral and ipsilateral-hit trials, respectively. Blue diamond, end of ipsilateral wheel rotation in the FA trial. Dashed line, end of timeout in the FA trial. c Averaged ΔF/F (z-scored) of an example PV+cal neuron in hit trials. Colored shading, ± SEM. Black dashed line, the start of visual white noise. Gray shading, window of the visuomotor period (from the start of drifting grating to the end of wheel rotation). d Color-coded averaged ΔF/F (z-scored) of all recorded PV+cal neurons in contralateral-hit (left) and ipsilateral-hit (right) trials (n = 8 mice, 8 sessions, 151 neurons). Each row shows the average activity of one neuron, sorted by peak response during the visuomotor period. Black dashed line, the start of visual white noise. The left and right gray dashed lines present the start of drifting grating and the end of wheel rotation, respectively. e Distribution of the difference in peak responses of z-scored ΔF/F during the visuomotor period between the contralateral-hit and ipsilateral-hit trials for the imaged PV+cal neurons. Blue, ipsilateral-preferring cells; red, contralateral-preferring cells. Orange arrowhead, median. There were significantly more ipsilateral-preferring PV+cal neurons than contralateral-preferring PV+cal neurons (P = 2 × 10−5, two-sided chi-square test). f Similar to c, but for all recorded PV+cal neurons. The averaged responses of PV+cal neurons were significantly stronger in the ipsilateral-hit trials than in the contralateral-hit trials during the visuomotor period (P = 3 × 10−5, two-sided paired t-test). See also Supplementary Fig. 12 and “Methods”. Source data are provided as a Source Data file. Created with BioRender.com.
