Fig. 1: Rationale behind QTG-Miner.
From: QTG-Miner aids rapid dissection of the genetic base of tassel branch number in maize
QTG-Miner integrates three procedures: 1) primary QTL mapping, 2) screening and sequencing on single QTL segregating material, and 3) candidate gene mining by QTG-Miner. In 1), mapping population types include but are not limited to F2, DHs, RILs, and NILs. In 2), single QTL-paired materials are screened, planted, and sampled for RNA-seq. Identified DEGs and sequence variants are considered as proportional weights during candidate gene prediction. In 3), SD and ML algorithms are integrated into QTG-Miner. Using SD and ML algorithms, candidate genes underlying the target QTL can be uncovered and subsequently verified by EMS mutagenesis or CRISPR/Cas9-mediated editing.
