Fig. 3: hTSCs lack intrinsic expression of representative antiviral ISGs.

a Venn diagram showing the number of ISGs specifically expressed in hTSCs, STB^TS and EVT^TS, and shared by all the cell types. b Heatmap showing the z-score TPM of ISGs with high and low expression in hTSCs, STB^TS and EVT^TS. c Quantification of the expression of ISGs with high and low expression shown by RNA-seq in hTSCs, STB^TS and EVT^TS by qRT-PCR. n = 3 independent experiments. Data are shown as the mean ± s.d. d PCA analysis of the transcriptomes of mock- and ZIKV-infected hTSCs, STB^TS and EVT^TS. The cells were exposed to ZIKV at an MOI of 1, and analyzed at 48 hours post infection. e Venn diagram showing the number of ISGs specifically up-regulated in hTSCs, STB^TS and EVT^TS following ZIKV infection and shared by all the cell types. f Heatmap showing the up-regulated ISGs in hTSCs, STB^TS and EVT^TS. g Quantification of the relative expression of ISGs in ZIKV-infected hTSCs compared to in mock-infected hTSCs by qRT-PCR. The hTSCs were infected with ZIKV at an MOI of 1 and the infected cells were separated by FACS for qRT-PCR analysis at 48 hours post infection. n = 3 independent experiments.