Fig. 2: Serotonin signaling is permissive in the ectoderm. | Nature Communications

Fig. 2: Serotonin signaling is permissive in the ectoderm.

From: Serotonin signaling regulates actomyosin contractility during morphogenesis in evolutionarily divergent lineages

Fig. 2

a Schematic of serotonin biogenesis, tryptophan hydroxylase (Trh) is the rate-limiting enzyme. b–d, b Snapshot of MyoII in Trh loss-of-function (Trh01 mutant); control (left panel) and Trh01 (right panel). Quantification of mean medial-apical MyoII levels over-time (c) and an example of the distribution of junctional MyoII intensities in different junctional orientations for different conditions (d). e–h Time-lapse of MyoII in Trh gain-of-function (Trh + +), top panels: control, lower panels: Trh + +, orange arrowheads indicate hyper-polarization of junctional MyoII (e). Quantification of MyoII intensities over-time in DV and AP oriented junctions (f), amplitude of polarity (g), and medial MyoII intensities (h). i–k Trh + + and 5HT2A−/−. i MyoII images in different conditions (from left to right respectively): control, Trh + + (orange arrowheads indicate hyper-polarization), 5HT2A-/- and 5HT2A-/- Trh + +. Quantification of junctional (j), and medial MyoII (k) intensities in different conditions. l–o Trh01 and 5HT2A + +. MyoII in control (left panel), 5HT2A + + (middle panel, orange arrowheads indicate hyper-polarization) and Trh01 5HT2A + + (right panel) (l), quantification of junctional MyoII intensities (m), amplitude of polarity (n), and medial MyoII (o) in different genotypes. In the box plots in (c, d, f, g, h, j, k, m, n, o), the line in the middle is plotted at the median. The box extends from the 25th to 75th percentiles. The whiskers indicate minimum and maximum values. Statistics: ns: P > 0.05, *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005, ****P ≤ 0.00005, *****P ≤ 0.000005 from two-tailed Mann–Whitney test. P values in (c): 1 (P = 0.01), 2 (P = 0.0004), 3 (P = 0.014), 4 (P = 0.0007). P values in (d): 1 (P = 0.375), 2 (P = 0.285), 3 (P = 0.596), 4 (P = 0.211), 5 (P = 0.328), 6 (P = 0.285). P values in (f): 1 (P = 0.013), 2 (P = 0.002), 3 (P = 0.000055), 4 (P = 0.022), 5 (P = 0.157), 6 (P = 0.238), 7 (P = 0.475), 8 (P = 0.475). P values in (g): 1 (P = 0.114), 2 (P = 0.024), 3 (P = 0.005), 4 (P = 0.03). P values in (h): 1 (P = 0.035), 2 (P = 0.711), 3 (P = 0.537), 4 (P = 0.786). P values in (j): 1 (P = 0.963), 2 (P = 0.134), 3 (P = 0.264), 4 (P = 0.43), 5 (P = 0.147), 6 (P = 0.029), 7 (P = 1.2 × 10−5), 8 (P = 0.01), 9 (P = 0.001), 10 (P = 2.26 × 10−4), 11 (P = 0.038), 12 (P = 0.056), 13 (P = 0.001), 14 (P = 3.8 × 10−4), 15 (P = 0.001), 16 (P = 1.7 × 10−4), 17 (P = 0.001), 18 (P = 0.005). P values in (k): 1 (P = 0.853), 2 (P = 1.2 × 10−5), 3 (P = 6 × 10−6), 4 (P = 0.842), 5 (P < 1 × 10−6). P values in (m): 1 (P = 0.694), 2 (P = 0.232), 3 (P = 0.04), 4 (P = 0.121), 5 (P = 0.006), 6 (P = 3.11 × 10−4), 7 (P = 0.008), 8 (P = 0.004), 9 (P = 0.004), 10 (P = 0.011), 11 (P = 4.4 × 10−4), 12 (P = 6.3 × 10−5), 13 (P = 0.237), 14 (P = 0.442), 15 (P = 0.6), 16 (P = 0.657), 17 (P = 0.6), 18 (P = 0.6). P values in (n): 1 (P= 0.009), 2 (P = 0.091), 3 (P = 0.104). P values in (o): 1 (P = 0.281), 2 (P = 0.0005), 3 (P = 0.006). n = number of embryos. Scale bars 5 µm. Source data are provided as a Source Data file.

Back to article page