Fig. 2: Analysis of reference eGFP mRNA vaccine using long-read Oxford Nanopore sequencing (PCS111). | Nature Communications

Fig. 2: Analysis of reference eGFP mRNA vaccine using long-read Oxford Nanopore sequencing (PCS111).

From: mRNA vaccine quality analysis using RNA sequencing

Fig. 2

a Genome-browser (IGV) view of long-read cDNA alignments to the reference plasmid sequence. Coverage indicates the number of reads at each nucleotide position while the lower alignments grey bars indicate unique, individual alignments, with colouring indicating their similarity to the reference genome. b Sequencing error and type profile across mRNA vaccine and poly(A) tail sequences. c Detail shows sequencing coverage and error at the poly(A) tail, showing a characteristic m-shaped deletion profile. d mRNA length as measured using ONT full-length sequencing shows full-length and fragmented mRNA vaccines. e poly(A) tail length measured using tailfindr for eGFP mRNA (green), compared to a cDNA library with variable poly(A) tail lengths. Source data are provided as a Source data file.

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