Fig. 2: Effects of PCPA and 5-HTP administration in infection.
From: Bridging of host-microbiota tryptophan partitioning by the serotonin pathway in fungal pneumonia
a Tph1+/+ and Tph1–/– littermates were infected intranasally with A. fumigatus and treated intraperitoneally with 5.5 mg/mouse of parachlorophenylalanine (PCPA) or 5-Hydroxytryptophan (5-HTP), respectively, 1 day before the infection and every other day post infection (dpi). Mice were monitored for b survival (%) (n = 12 mice/group), c fungal burden (n = 5 mice/group), and d neutrophils (%) in the bronco-alveolar lavage (n = 3–4 mice/group), e lung histology (periodic acid-Schiff staining) and f cytokine production in lung homogenates (ELISA) at 7 dpi (n = 3 in Tph1+/+ and n = 4 in Tph1–/–mice/group). Photographs were taken with a high-resolution microscope (Olympus BX51), ×10 magnification (scale bar 500 µm). Data are expressed as mean ± SEM and are representative of two experiments. *p < 0.05, **p < 0.01, and ***p < 0.001. b Log-rank (Mantel-Cox) test. P value 0.0319. c–f Unpaired t test, two-tailed. P values in c: 0.0300 (Tph1+/+), 0.017 (Tph1–/–); in d: 0.0236; in f: for IL-1β, 0.0065 (Tph1+/+), 0.0002 (Tph1–/–); for IL-17A, 0.0008; for TNF-α, 0.0185 (Tph1+/+), 0.0141 (Tph1–/–). Source Data are provided as a Source Data file.
