Fig. 4: EGGD-1 is required for piRNA-mediated gene silencing, but dispensable for gene silencing by dsRNAs.
From: C. elegans germ granules sculpt both germline and somatic RNAome
a Representative single plane confocal images of the piRNA reporter strain when exposed to control (top) and snpc-4 (bottom) RNAi food. n for each RNAi condition was shown in Fig. 4b. Scale bar = 10 μm. b Table summarizing the percentage of worms with reporter ON for each RNAi treatment. n = worms imaged over one experiment. c Bar graph showing mean ± standard deviation percentage of embryos hatched for wild-type, eggd-1, eggd-2, and eggd-1; eggd-2 worms plated on L4440 (control) or pos-1 RNAi (n = 3 independent experiments). rde-1 dataset was collected from one experiment. For each experiment, n ≥ 200 embryos assayed for viability. Source data are provided as a Source Data file. d Line graph showing GFP RNAi inheritance in three independent experiments for eggd-1, eggd-2, and wild-type worms over eight successive generations. Error bars indicate mean ± standard deviation. 50 worms per line were imaged each generation to determine GFP silencing status. Source data are provided as a Source Data file.
