Fig. 6: Long-term immunity test. | Nature Communications

Fig. 6: Long-term immunity test.

From: mRNA vaccines encoding fusion proteins of monkeypox virus antigens protect mice from vaccinia virus challenge

Fig. 6

Mice were divided into four groups and intramuscularly vaccinated with VGPox1, VGPox2, VGPox3 (10 µg/dose, twice on day 0 and day 14), or intraperitoneally injected with live virus VACV-WR (2 × 105 PFU/dose). Blood was collected monthly after the first dose vaccination for analysis of anti-A35R (a), anti-M1R (b) using ELISA as described previously and neutralizing antibodies (c). The sera from each mouse were 1 in 500 diluted and incubated with VACV-WR virus for VACV PRNT. Sera from the DPBS group were regarded as 0% neutralization. n = 8 biologically independent mice. Data are presented as mean values ± SD. After 5 months, mice were challenged intranasally with 1 × 106 PFU VACV-WR, and their body weight was recorded daily (d). n = 8 biologically independent mice.

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