Fig. 4: Sac1 binding to the SPOT complex.
a Sac1 interactions within the SPOTS complex. Color code is the same as in Fig. 1. b, c Close-up views of the interactions between Sac1 and Lcb2. Polar contacts are indicated with yellow dotted lines. The subunits are depicted as cartoons and ligands are shown in ball-and-stick representation. d Levels of 3-ketosphinganine (3-KS), long chain bases (LCBs), and ceramides in sac1Δ cells harboring a plasmid expressing ALFA-Sac1, sac1Δ cells or sac1Δ cells harboring a plasmid expressing ALFA-sac1Δ574. Data were analyzed by one-way ANOVA with Tukey’s multiple-comparison test (*p < 0.05, **p < 0.01, ***p < 0.001) with n = 4 biologically independent samples and data are presented as mean values ± SD. Exact P-values are shown in Sup. Tab. 7. e Label free proteomics of yeast cells expressing ALFA-Sac1 compared to untagged control cells. In the volcano plot, the protein abundance ratios of ALFA-Sac1 over control cells are plotted against the negative log10 of the P-value of the two-tailed t-test for each protein. f Label free proteomics of yeast cells expressing ALFA-sac1Δ574 compared to untagged control cells. In the volcano plot, the protein abundance ratios of ALFA-sac1Δ574 over control cells are plotted against the negative log10 of the P-value of the two-tailed t-test for each protein. g Label free proteomics of D. discoideum cells expressing GFP-Sac1 compared to untagged control cells. In the volcano plot, the protein abundance ratios of GFP-Sac1 over control cells are plotted against the negative log10 of the P-value of the two-tailed t-test for each protein. Source data are provided as a Source Data file for d, e, and f.
