Fig. 2: Longitudinal 16S analysis of microbiota shows early and persistent changes in composition of microbiota after FMT. | Nature Communications

Fig. 2: Longitudinal 16S analysis of microbiota shows early and persistent changes in composition of microbiota after FMT.

From: Immune checkpoint inhibitor-induced colitis is mediated by polyfunctional lymphocytes and is dependent on an IL23/IFNγ axis

Fig. 2

a Alpha-diversity (Shannon diversity index) of the microbiota between untreated control Balb/C wildtype female 6-week-old mice (n = 6), Balb/C wildtype mice treated with FMT (n = 6) and Balb/C wildtype female 6-week-old mice treated with CPI + FMT (n = 6) longitudinally over a 3 week period. A liner mixed model was used, based on ANCOM-BC, to account for the repeated measures of the mice. FDR Bonferroni corrected Padj. Box plot is defined by: Week 0 – Untreated = “Min.:3.775” “1st Qu.:3.787” “Median :3.828” “Mean:3.856” “3rd Qu.:3.867” “Max.:4.051” Week 1 – Untreated = “Min. :3.640” “1st Qu.:3.670” “Median :3.819” “Mean :3.909” “3rd Qu.:3.929” “Max. :4.586” Week 2 – Untreated =“Min. :3.659” “1st Qu.:3.823” “Median :3.871” “Mean :3.911” “3rd Qu.:3.968” “Max. :4.255“Week 3 – Untreated =“Min. :3.148” “1st Qu.:4.105” “Median :4.236” “Mean :4.049” “3rd Qu.:4.331” “Max. :4.427” Week 0 – FMT-only = “Min.:3.603” “1st Qu.:3.823” “Median:3.852” “Mean :3.890” “3rd Qu.:4.033” “Max. :4.137” Week 1 – FMT-only = “Min. :3.610” “1st Qu.:3.901” “Median :3.990” “Mean :4.007” “3rd Qu.:4.100” “Max. :4.445” Week 2 – FMT-only = “Min. :3.859” “1st Qu.:3.927” “Median :4.008” “Mean :4.021” “3rd Qu.:4.114” “Max. :4.206” Week 3 – FMT-only = “Min.:3.832” “1st Qu.:4.016” “Median :4.037” “Mean :4.069” “3rd Qu.:4.193” “Max. :4.250” Week 0 – CPI = “Min. :3.591” “1st Qu.:3.734” “Median :3.801” “Mean :3.772” “3rd Qu.:3.836” “Max. :3.878” Week 1 – CPI= “Min. :3.080” “1st Qu.:3.265” “Median :3.515” “Mean :3.557” “3rd Qu.:3.844” “Max. :4.097” Week 2 – CPI= “Min. :3.770” “1st Qu.:3.952” “Median :4.030” “Mean :4.084” “3rd Qu.:4.280” “Max. :4.384” Week 3 – CPI = “Min. :3.902” “1st Qu.:3.944” “Median :4.461” “Mean :4.348” “3rd Qu.:4.592” “Max. :4.839” b Non-metric dimensional scaling plot showing the beta diversity of the microbiota from untreated control Balb/C wildtype female 6-week-old mice (n = 6), Balb/C wildtype female 6-week-old mice treated with FMT (n = 6) and Balb/C wildtype female 6-week-old mice treated with CPI + FMT (n = 6) longitudinally over a 3 week period. c Phylum level relative abundance profiles from untreated control Balb/C wildtype female 6-week-old mice (n = 6), Balb/C wildtype female 6-week-old mice treated with FMT (n = 6) and Balb/C wildtype female 6-week-old mice treated with CPI + FMT (n = 6) longitudinally over a 3 week period. d Family level relative abundance profiles from untreated control Balb/C wildtype female 6-week-old mice (n = 6), Balb/C wildtype female 6-week-old mice treated with FMT (n = 6) and Balb/C wildtype female 6-week-old mice treated with CPI + FMT (n = 6) longitudinally over a 3 week period. Genus level coefficient changes comparing Balb/C wildtype female 6-week-old mice treated with FMT (n = 6) and Balb/C wildtype female 6-week-old mice treated with CPI + FMT (n = 6) at e 1 week, f 2 weeks and g 3 weeks of treatment. A liner mixed model was used, based on ANCOM-BC, to account for the repeated measures of the mice. FDR Bonferroni corrected Padj.

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