Fig. 2: FGF18 overexpression alleviates liver damage during hepatic IRI. | Nature Communications

Fig. 2: FGF18 overexpression alleviates liver damage during hepatic IRI.

From: FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice

Fig. 2

Mice were injected with AAV2/9-FGF18 via tail tein. After stably expressed for two weeks, mice were subjected to I/R (1 h/6 h) surgery. A Schematic diagram of the work and western blotting showed the transfection efficiency of AAV-FGF18 (n = 5). (Scheme is Created with BioRender.com) B Serum ALT and AST level (n = 5). C H&E staining of liver sections. Scale bar=100 μm (n = 5). D–F TUNEL staining, c-CAS-3 immunohistochemistry, and MPO staining of liver sections (n = 5). Scale bar = 100 μm. G Relative protein expression by western blotting (n = 3). H Protein levels of apoptosis related signaling pathway in H/R (4 h/6 h) treated L02 cells in the presence of FGF18 (200 ng/mL) subjected to challenge or not (n = 3). I, J Relative mRNA expression of genes by RT-PCR (n = 3). H/R, hypoxia/reoxygenation; The statistical significance of differences were assessed by two-tailed two-tailed student unpaired t-test for C–F. Other assays were assessed by one-way ANOVA, followed by Tukey’s multiple comparison test. Data are presented as means ± SEM with individual values. All numbers (n) are biologically independent experiments. Source data are provided as a Source Data file.

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