Fig. 7: USP16 is essential for aggravation of hepatic IRI by FGF18 deficiency. | Nature Communications

Fig. 7: USP16 is essential for aggravation of hepatic IRI by FGF18 deficiency.

From: FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice

Fig. 7

Fgf18△HSC mice were injected with AAV8-TBG-sh-USP16 via tail tein. After stably expressed for two weeks, mice were subjected to I/R (1 h/6 h) surgery. A Schematic diagram of the work (n = 5) (Scheme is Created with BioRender.com). B Serum ALT and AST level (n = 5). C H&E staining of liver sections. Scale bar = 100 μm. D–G TUNEL staining, c-CAS-3 immunohistochemistry, MPO, and 4-HNE staining of liver sections (n = 5). Scale bar = 100 μm. H Relative protein expression by western blotting (n = 3). I Liver GSH and MDA levels (n = 5). The statistical significance of differences were assessed by two-tailed student unpaired t-test. Data are presented as means ± SEM with individual values. All numbers (n) are biologically independent experiments. Source data are provided as a Source Data file.

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