Fig. 8: FGF18-induced Nrf2 binds to the USP16 promoter and forms a negative feedback loop. | Nature Communications

Fig. 8: FGF18-induced Nrf2 binds to the USP16 promoter and forms a negative feedback loop.

From: FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice

Fig. 8

A Protein levels of USP16 and HO-1 in H/R (4 h/6 h) treated HepG2 cells in the presence or absence of FGF18 or ML385 (10 μM), or not (n = 3 per group) (n = 3). B Relative mRNA expression of genes by RT-PCR (n = 3). C Relative protein expression by western blotting (n = 3). D Relative mRNA expression of genes by RT-PCR (n = 3). E Relative luciferase activity was detected (n = 3). F Binding of Nrf2 to the USP16 promoter region in H/R treated HepG2 cells was examined by CHIP assay (n = 3). The statistical significance of differences were assessed by two-tailed student unpaired t-test for (C, D). Other assays were assessed by one-way ANOVA, followed by Tukey’s multiple comparison test. Data are presented as means ± SEM with individual values. All numbers (n) are biologically independent experiments. Source data are provided as a Source Data file.

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