Fig. 4: Functional validation of Lr47 by BSMV-mediated gene editing and transgenic complementation.
From: Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
a Sequencing chromatogram showing the induced polymorphisms between the wild type (WT) and the selected BSMV-Cas9-induced editing mutants. The mutated nucleotides are underlined and the CCT PAM sequence is highlighted in red. b Reactions to Pt race THDB. (1) Kern Lr47; (2, 3) mutant line mut-1; (4, 5) mutant line mut-2; and (6) Cas9-transgenic Bobwhite. c The 7,234-bp genomic DNA fragment including Lr47 used for transformation. The black arrow indicates the Lr47 gene (from ATG to TGA). d Infection types of two transgenic families T1CS401-1 and T1CS401-2 and Fielder control in response to Pt races FHJR, PHRT, PHTT, PHQS, FHJL, HCJR, and a mixture of naturally prevalent Pt races collected from the field in 2021 (field race, 36°26′04.0″N, 119°26′42.6″E). Copy number of transgenes was estimated using TaqMan assays (Supplementary Table 3). R resistant, S susceptible. Source data are provided as a Source data file.
