Fig. 5: Reduction in the length of the introgressed Ae. speltoides chromosome segment carrying Lr47.
From: Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
a Infection types of homozygous BC1F2 plants from the introgression line YM21 + Lr47-2 (+) and the plants lacking Lr47 (-). Six Pt races (THDB, PHRT, PHTT, PHQS, FHJL, and HCJR) were used for evaluation. R, Resistant; S, Susceptible. b–d GISH images of wheat lines Kern Lr47, L2 (BC2F2), and L8 (BC1F2). The magnified images show the Ae. speltoides chromosome segments introgressed into wheat chromosome 7A (Ae. speltoides chromatin is painted in green and marked with red arrowheads). e–g FISH images from wheat lines Kern Lr47, L2 (BC2F2), and L8 (BC1F2). Probes pSc119.2 (green), pTa535 (red), and pTa713 (yellow) were used. Yellow arrows indicate the wheat-Ae. speltoides translocated 7A chromosomes, and magnified images of these chromosomes are shown in the insets. Green arrows indicate the 4AL/7BS translocation. Scale bar = 10 μm. All experiments were repeated three times independently with consistent results. Source data are provided as a Source data file.
