Fig. 6: Statistical analysis of agronomic and quality traits in YM21 + Lr47-1 and its sister control line.

a–p Plants grown in a controlled walk-in growth chamber at 24 °C day/22 °C night with a 16 h light/8 h dark photoperiod were phenotyped for the following traits: a plant height (PH); b tillers number (TN); c spike length (SL); d spikelet number per spike (SNS); e grain number per spike (GNS); f grain yield per plant (GYP); g thousand-seed weight (TSW); h grain length (GL); i grain width (GW); j shearing force (SF) in Newtons (N); k grain moisture content (GMC); l grain protein content (GPC); m flour water absorption (FWA); n flour ash (FA); o flour yield (FY); and p grain hardness (GH). q–s Plants grown in the field. q Visual phenotypes of YM21 + Lr47-1 and its sister line in the field. r Infection types of YM21 + Lr47-1 and the sister control line. s Spike length (SL). In each box plot, the horizontal line corresponds to the median score, box edges show the first and third quartiles, and whiskers extend to the maximum and minimum values within 1.5 times the interquartile range. The shape of the violin plot reflects the distribution of the variable. Black triangles represent single data points. The significance of the differences was estimated using two-sided unpaired t test. ns not significant (P > 0.05), *P < 0.05. Lr47, resistant Lr47 allele present (YM21 + Lr47-1); lr47, sister control line lacking the alien chromosome segment. Source data are provided as a Source data file.