Fig. 1: Constructs built and transgenic mosquitoes generated to test a split-drive system in Culex quinquefasciatus targeting the white locus. | Nature Communications

Fig. 1: Constructs built and transgenic mosquitoes generated to test a split-drive system in Culex quinquefasciatus targeting the white locus.

From: CRISPR-based gene drives generate super-Mendelian inheritance in the disease vector Culex quinquefasciatus

Fig. 1

a Schematic outline of targeted gene locations in Cx. quinquefasciatus chromosome I. The approximate distances between different loci are indicated. The approximate ___location of the sex-determining locus is indicated with a black bar. Drawings are not to scale. b Graphical representation of the plasmid construct and transgene integration strategy used to build the vasa-Cas9 line in our previous study26. c The gRNA-eGFP construct and transgene integration strategy for building the white-gRNA6 homing element line. b Pink and c gray shading highlight homology arms (HAs) correspondence to the genome sequences used for site-directed integration. The w4- mutation marked with a magenta asterisk (*) sits a distance of 5.3 kb from the white-gRNA6 target site located on exon 5. Graphs are not to scale. d Photo of a transgenic adult mosquito expressing eGFP (left) compared to a wildtype mosquito (right). e Photo of three larvae: left, vasa-Cas9 line expressing DsRed; middle, white-gRNA6 line expressing eGFP; right, a wildtype larvae without fluorophore expression.

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