Fig. 4: A split gene drive targeting the kmo gene in Culex quinquefasciatus. | Nature Communications

Fig. 4: A split gene drive targeting the kmo gene in Culex quinquefasciatus.

From: CRISPR-based gene drives generate super-Mendelian inheritance in the disease vector Culex quinquefasciatus

Fig. 4

a Genetic cross scheme for the experiment. G0 individuals carrying the kmo-gRNA transgene were reciprocally mated en masse to individuals carrying the vasa-Cas9 transgene. Male and female transheterozygote progeny from each of these two crosses were mated en masse to wildtype of the opposite sex (G1). Inheritance of vasa-Cas9 and kmo-gRNA transgenes scored in their progeny (G2). The different phenotypes of G2 individuals are listed. Potential allelic conversion events (homing) are indicated by blue inverted triangles in the cross scheme. b The inheritance rates of kmo-gRNA (marked by Hr5IE1-DsRed) and vasa-Cas9 (marked by Opie2-DsRed) transgenes in the G2 progeny. Estimated means and 95% confidence intervals were calculated by a generalized linear mixed model, with a binomial (‘logit’ link) error distribution, and are presented above the graphs for each data set. Raw data points are given in the graph whereas modeled data is provided in the main text. Raw phenotypic scoring is provided in Supplementary Data 3.

Back to article page