Fig. 2: LC-MS analysis of the products formed by MonCI in the absence and presence of an NADH regeneration system.
From: Triepoxide formation by a flavin-dependent monooxygenase in monensin biosynthesis
a Reaction mixture containing 160 µM farnesyl 4-hydroxybenzoate (FHB), 4 mM NADH, 15 µM Fre, 300 mM NaCl, 10% (v/v) glycerol, and 15 µM MonCI in 50 mM Tris, pH 8.0 was incubated for six hours at 30 °C prior to LC-MS analysis. b Reaction mixture containing 160 µM farnesyl 4-hydroxybenzoate, 15 µM glucose dehydrogenase, 80 mM d-glucose, 4 mM NAD+, 15 µM Fre, 300 mM NaCl, 10% (v/v) glycerol, and 15 µM MonCI in 50 mM Tris, pH 8.0 was incubated for 6 h at 30 °C prior to LC-MS analysis. Source data are provided as a Source Data file. Mass spectrum corresponding to individual peaks are presented in Fig. 3.
