Fig. 5: JEDI-1P-Kv signals correlate with local field potentials (LFPs).
a Setup schematic for simultaneous voltage imaging and acute LFP recording in lightly anesthetized animals. Lines indicate the cortical map derived from the Allen Mouse Brain Common Coordinate Framework92. M1, primary motor cortex. M2, secondary motor cortex; S1-bfd, primary somatosensory (barrel) cortex; RSPd, retrosplenial cortex; V1, primary visual cortex. b JEDI-1P-Kv reports voltage transients also seen in LFP traces. Voltage imaging was conducted close (ROI 1i in (c)) to the LFP recording site (asterisk in (c)). LFPs were recorded from 32 channels, with inverted traces from 4 channels shown here (channels 1, 9, 17, 25, and 31). Depths are relative to the top channel. c, d LFPs are correlated with JEDI-1P-Kv signals from both ipsilateral (i) and contralateral (c) sides. c Map showing the mean correlation between the LFP recording and JEDI-1P-Kv signals from 2 × 2-pixel ROIs in a representative mouse. The purple asterisk indicates the LFP electrode insertion site. n = 40 trials. d LFP and JEDI-1P-Kv signals are correlated at each ROI (p = 7.6 × 10−42, 5.1 × 10−39, 1.3 × 10−9, 4.3 × 10−9, 1.5 × 10−34, 6.9 × 10−40, 2.0 × 10−8, 1.9 × 10−11, two-sided Mann–Whiteney U test). For both groups at each ROI, n = 172 trials from 3 mice, with 40-84 trials/mouse. Shuffled control correlation coefficients are calculated from mismatched LFP recording and imaging trials. ROI locations are shown in (c). Dots are individual trials, the horizontal lines are the mean value, and the error bars are the 95% confidence intervals. e LFP and JEDI-1P-Kv signals are correlated below 60 Hz based on the magnitude-squared coherence. Note that the dip in the coherence at 60 Hz is expected due to the LFP notch filter at this frequency. Dark lines are the mean, and shaded areas are the 95% confidence intervals. Panel a was created using Biorender.com.
