Fig. 6: Characterization of CD31-CD34+ stromal cells and cell‒cell communication analyzed by scRNA-seq. | Nature Communications

Fig. 6: Characterization of CD31-CD34+ stromal cells and cell‒cell communication analyzed by scRNA-seq.

From: Fibroblast growth factor 18 stimulates the proliferation of hepatic stellate cells, thereby inducing liver fibrosis

Fig. 6

a UMAP plot showing the 22 clusters of nonparenchymal liver cells from 6-week-old non-Tg and Fgf18 Tg mice (n = 3 mice per genotype). Each number indicates each cell cluster. b Relative percentages of each cluster from non-Tg and Fgf18 Tg mice are shown. Clusters containing HSC, portal fibroblast, and myofibroblast are indicated by the white boxes. c Violin plot showing the expression levels of the indicated genes in HSCs, portal fibroblasts, and myofibroblasts pooled from non-Tg and Fgf18 Tg mice. d Circle plot showing the expression levels and percentages of cells expressing the indicated genes from non-Tg and Fgf18 Tg mice. Color intensities and circle sizes indicate expression levels and percentages of cells expressing the indicated genes, respectively. e Communication networks between HSCs/fibroblasts and other cells as analyzed by CellChat. Notably, FGF18 signals appeared to originate from various types of cells; this may be caused by read-through transcripts of Fgf18 expressed in multiple tissues. All significant ligand‒receptor pairs that contribute to sending signals from HSC and fibroblast to other cells are shown. The edge width represents the communication probability. scRNA-seq data for endothelial cell and cholangiocyte were not included in the present data. M-FIB myofibroblasts, P-FIB portal fibroblast, HSC hepatic stellate cell, Hep hepatocyte, DC dendritic cell, Mo monocyte, Neu neutrophil.

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