Fig. 3: Unraveling invisible morphological variation in a patient-derived dopaminergic neuron assay.
From: Revealing invisible cell phenotypes with conditional generative modeling
a IPSCs are derived from fibroblasts sampled from a LRRK2 G2019S mutated Parkinson’s patient. These IPScs are then reprogrammed to dopaminergic neurons with or without a CRISPR-cas9 correction of the G2019S mutation. The latter is an isogenic engineered wild type. Large sets of confocal images with one dye labeling for Nuclei, and antibody stains labeling for alpha-synuclein and TH cells, scale bar is 20 μm. Real images display no detectable visual systematic differences. b A conditional GAN trained in order to identify differences between these two close conditions displays 1 - an increase of dopaminergic neurons and dendritic complexity in the engineered WT condition, 2 - removal of some of the IPScs in the WT condition, 3- alpha-synuclein seems to shift from the IPSc cytoplasm to the nuclei before it eventually decreases in fully shaped differentiated WT neurons (more examples here https://www.phenexplain.bio.ens.psl.eu/lrrk2.html).
