Fig. 2: Preparation and characterization of biNV-IL-15. | Nature Communications

Fig. 2: Preparation and characterization of biNV-IL-15.

From: Biomimetic nanovaccine-mediated multivalent IL-15 self-transpresentation (MIST) for potent and safe cancer immunotherapy

Fig. 2

a Production of biNV-IL-15 isolated from recombinant adenovirus-transduced DCs. (1) Adenovirus vectors were genetically modified with IL-15/IL-15Rα gene. (2) Recombinant adenovirus transduced the BMDCs to express membrane-bound IL-15 on the cell surface. (3) Tumor antigen priming and DC maturation. (4) The isolation of biNV-IL-15. b Schematic representation of the production of biNV-IL-15. c The comparison of HSP70 expression between normal tissue (NT) and tumor tissue (TT) on 4T1 and CT26 tumors through immunohistochemistry. Scale bar = 100 μm. d The diameter of extracted HCP measured by DLS. e SDS-PAGE protein analysis of the isolated HCP and 4T1, CT26 tumors. f TEM images of biNV-IL-15. Inset: immunogold labeling of MHC-I (10 nm gold particle). Scale bar = 100 nm. g NTA analysis of biNV-IL-15. h CLSM images of MHC-I (red) and IL-15 (green) on biNV-IL-15. Scale bar = 1 μm. i Western blotting analysis of B7-1, B7-2, ICAM-I, CCR7, IL-15, and IL-15Rα on biNV-IL-15. For panels c, e, f, h, experiment was repeated three times independently with similar results. For panel i, experiment was repeated twice independently with similar results. Source data underlying panel g, i are provided as a Source Data file.

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