Fig. 3: LNP mediated photoreceptor co-localization in Ai9 retina. | Nature Communications

Fig. 3: LNP mediated photoreceptor co-localization in Ai9 retina.

From: Lipid nanoparticles with PEG-variant surface modifications mediate genome editing in the mouse retina

Fig. 3

Representative confocal microscopy images of the retinal sections showing tdTomato (red), stained with visual arrestin (green; rods, cones, and outer segments) and DAPI (blue) for (A) PBS, (B) LNPz, and (C) LNPx treatment. D A low magnified confocal image of an LNPx treated retina showing pan-retinal tdTomato expression from the photoreceptor synapse to the outer segment in both rod and cone photoreceptors. E tdTomato fluorescence signal (red) in the photoreceptors was co-localized with recoverin (green) for PBS and LNPx treatment. 2 to 3-month-old mice were used. All representative figures from n  =  6 single eye each group. Arrow indicates tdTomato expression (pink: RPE, yellow: cone photoreceptor nuclei, and green: rod photoreceptor nuclei). RPE retinal pigment epithelium and ONL outer nuclear layer.

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