Fig. 2: Optimization of leader/anchor combination for cell-surface expression.
From: Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging

a Overview of biosensor optimization. b Localization of eLACCO1.1 with CD59 leader sequence and different anchors in rat primary neurons. Scale bars, 200 μm. Right panels show magnified images in the presence of l-lactate. Scale bars, 50 μm. c Localization index and ΔF/F of eLACCO1.1 with CD59 leader sequence and different anchors in rat primary neurons. Mean ± s.e.m., n = 3 field of views (FOVs) over three wells per construct. d Localization of eLACCO1.1 with different leader sequences and NGR GPI anchor in rat primary neurons in the presence of l-lactate. Scale bars, 200 μm. e Localization index and neurite brightness of eLACCO1.1 with different leader sequences and NGR GPI anchor in rat primary neurons. Mean ± s.e.m., n = 9 FOVs over three wells per construct. Source data are provided as a Source Data file.