Fig. 2: NF186 proximity proteomes across neuronal development. | Nature Communications

Fig. 2: NF186 proximity proteomes across neuronal development.

From: Antibody-directed extracellular proximity biotinylation reveals that Contactin-1 regulates axo-axonic innervation of axon initial segments

Fig. 2

a Immunofluorescence labeling of NF186 at different timepoints of hippocampal neuron development in vitro; N = 3 independent experiments. Lower panels: magnified images show the Nfasc-labeled AIS at each time point. Scale bars, 20 μm. b–f Volcano plots showing the log2-fold changes of proteins versus the statistical significance -log10(pvalue) identified using Nfasc-directed proximity biotinylation (N = 3). P values were calculated using a nonparametric two-sided T-test; no adjustments were made for multiple comparisons. P < 0.05 was used as a cutoff for significance (horizontal dashed line). Some identified proteins are indicated (corresponding gene names listed); those previously reported as AIS cell surface proteins are shown in red.

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