Fig. 6: RNF13 inhibits STING-mediated inflammatory signaling pathways in NASH.

KEGG (a) analysis and GSEA (b) showing the results of RNA sequencing using the liver tissues from Rnf13Flox/Flox and Rnf13HKO mice after 16-week HFHC feeding. The Indicated protein levels in the livers of HFHC-induced Rnf13Flox/Flox and Rnf13HKO mice (c), and Rnf13NTg and Rnf13HepTg mice (d) (n = 3). The Indicated protein levels in MPHs infected with AdshRnf13 (e) and AdRnf13 (f) as well as the corresponding control viruses followed by PAOA treatment for 12 h (n = 3). Endogenous STING protein level in MPHs (g) and HepG2 cells (h) in response to different doses of RNF13 overexpression. qPCR analyses of Sting1 mRNA in MPHs infected with AdshRnf13 (i) and AdRnf13 (j) as well as their corresponding control viruses, followed by PAOA treatment for 12 h (n = 5). The indicated protein levels (k), Nile Red staining and quantification (l), TG contents (m), lipogenic (n) and proinflammatory gene expression (o) in MPHs infected with AdGFP, AdRnf13 or AdRnf13 plus AdSting1 with PAOA treatment for 12 h (For k–m, n = 3; for n–o, n = 4). Scale bars, 25μm. Data were expressed as mean ± SD. Two-tailed Student’s t-test for i and j, one-way ANOVA with Bonferroni post hoc analysis for l–o. Source data are provided as a Source Data file.