Fig. 1: Molecular and structural investigation of β-lg monomers and amyloid fibrils during in-vitro gastrointestinal digestion.
From: Food amyloid fibrils are safe nutrition ingredients based on in-vitro and in-vivo assessment
a Simulated gastrointestinal digestion phases of interest. This figure was partly generated using Servier Medical Art provided by Servier licensed under a Creative Commons Attribution 3.0 unported license [https://smart.servier.com/how-to-cite-servier-medical-art/]. b, c SDS-PAGE of β-lg monomer (b) and amyloid fibrils (c). The red arrows indicate the β-lg monomer (b) and amyloid fibrils (c). d, e ELISA antigenicity assay of β-lg monomer (d) and amyloid fibrils (e). The boxes cover the range of 25–75 percentage, the whisker lines and center lines refer to SD and median value respectively and square symbols indicate the mean value. n = 3 independent experiments. f, g TO fluorescence assay of β-lg monomer (f) and amyloid fibrils (g). The enzymatic signal refers to background contribution from digestive enzymes. n = 3 independent experiments. h, i CD spectra of β-lg monomer (h) and amyloid fibrils (i) before and after digestion.
