Fig. 5: In-vivo effects of amyloid ingestion on C. elegans. | Nature Communications

Fig. 5: In-vivo effects of amyloid ingestion on C. elegans.

From: Food amyloid fibrils are safe nutrition ingredients based on in-vitro and in-vivo assessment

Fig. 5

a No mobility impairment in any condition after ingestion of either digested or undigested lysozyme and β-lg monomers and fibrils. By contrast, β-lg fibrils and lysozyme fibrils prolonged healthspan (active movement) of C. elegans. Data was analysed using a one-way ANOVA, followed by Dunnett’s multiple comparisons test of the area under the curve. Proteins were supplemented at day 1 of adulthood. Data are presented as mean ± SD. The p value of the control vs. β-lg samples are β-lg monomer (0,9546), β-lg mono digested (0,9918), β-lg fibril (<0,0001) and β-lg fibril digested (0,9996). The p value of the control vs. lysozyme samples are lysozyme monomer (0,9842), lysozyme mono digested (>0,9999), lysozyme fibril (<0,0001), lysozyme digested (0,3958) and digest buffer control (0,9996). b, c Representative transgenic AM140 C. elegans showing of non-aggregated (day 1) (b) and aggregated (day 5) (c) Q35-YFP signal, respectively. N = 3 biological replicates. d Supplementation of protein at day 1 of adulthood leads to increased number of polyglutamine (Q35) aggregates in transgenic AM140 C. elegans expressing Q35-YFP in muscles ingesting a high concentration of lysozyme fibrils, but no other condition. Shown is one out of three independent biological trials. Data was analysed using a one-way ANOVA, followed by Dunnett’s multiple comparisons test. Data are presented as mean ± SD.

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