Fig. 2: Novel isoforms and genes are broadly expressed in early preimplantation stages.

A Overview of the data types integrated and approach to validate the novel isoform-resolved transcriptome. B Percentage of isoforms in each class, grouped by the number of integrated short-read RNA-Seq datasets in which they are expressed (Yan et al., Liu et al., Xue et al.). C Percentage of isoforms in each developmental stage, grouped by isoform class and average expression level across short-read RNA-Seq datasets profiling human preimplantation embryos and oocytes (TPM – Transcript Per Million). D As above, but displaying data from single-cell short-read RNA-Seq datasets (SmartSeq2) profiling in-vitro models of human preimplantation development (8CLCs and blastoids). Source data are provided as a Source Data file. Icons in Fig. 2A, C were created with BioRender.com.