Fig. 1: Dynamic changes in the transcriptomes and epi-transcriptomes of PBMCs in irradiated mice.

a Body weight of 6–8 weeks old C57BL/6 male mice after total-body irradiation (TBI) by gamma rays at different doses (n = 5 for each time point). Data are presented as means ± standard deviation (SD). b Schematic for a two-stage screening of N6-methyladenosine (m⁶A) modification responsive to gamma-ray radiation in peripheral blood mononuclear cells (PBMCs) from the mice exposed to gamma-ray radiation (n = 5 for each time point). c The significantly up-regulated genes and hyper-m⁶A methylated genes in PBMCs from mice at multiple time points (days 1, 3, 7, 14, and 30) after 6.5 Gy gamma-ray radiation. GE: gene expression profile. d The significantly down-regulated genes and hypo-m⁶A methylated genes in PBMCs from mice at multiple time points (days 1, 3, 7, 14, and 30) after 6.5 Gy gamma-ray radiation. The sub-categories of hyper- (e) and hypo-methylated (f) RNAs identified by unsupervised clustering analyses. X axis represents the time post irradiation, Y axis represents the log-transformed fold changes of m⁶A levels. Each line represents the dynamic changes of the m⁶A levels of each RNA transcript, and the membership of lines is represented by colors. g The significantly up-regulated genes and hyper-m⁶A methylated genes (left panel) and down-regulated genes and hypo-m⁶A methylated genes (right panel) in response to 2 Gy gamma-ray radiation at two time points (day 1 and 14) in mice PBMCs. h Venn diagram showing the overlaps among three RNA sets: consistently hyper-m⁶A methylated RNAs after 6.5 Gy irradiation (Hyper_6.5Gy_consis), hyper-m⁶A methylated RNAs after 2 Gy irradiation at day 1 (Hyper_2Gy_D1), and hyper-m⁶A methylated RNAs after 2 Gy irradiation at day 14 (Hyper_2Gy_D14). i A heat map showing the m⁶A modification levels of Ncoa4, Ate1, and Fgf22 mRNAs across all the time points in stages I (days 1, 3, 7, 14, and 30) and II (days 1 and 14) experiments. Colors represent logFC of m⁶A levels between PBMCs from the irradiated mice and control mice. Source data are provided.