Fig. 2: Myofiber-specific ablation of FNIP1 promotes the formation of patent, functional blood vessels. | Nature Communications

Fig. 2: Myofiber-specific ablation of FNIP1 promotes the formation of patent, functional blood vessels.

From: FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment

Fig. 2

a Representative hindlimbs from Fnip1f/f and FNIP1 MKO mice at the age of 8 weeks. b Volcano plot showing fold changes versus P values for analyzed RNA-seq data generated from the GC muscles of 8-week-old male FNIP1 MKO mice compared with Fnip1f/f littermate controls. Significantly upregulated genes are represented by red dots, whereas downregulated genes are represented by blue dots. c GO enrichment analysis (“Biological Process_Direct” term) of gene transcripts upregulated in FNIP1 MKO muscle. d Representative images of CD31 immunofluorescent (green) and microsphere perfused (red) images in GC muscles from 14-week-old Fnip1f/f and FNIP1 MKO mice. Scale bar, 100 µm. e Quantification of capillaries per mm2 in (d). n = 6 biologically independent mice per group. P value: 0.0001, <0.0001. f Representative confocal images of immunostainings of CD31 (green) and PDGFRβ (red) co-staining in Fnip1f/f and FNIP1 MKO GC muscles. Scale bar, 100 µm. g Pearson’s correlation of pericyte density (PDGFRβ) and Capillary density (CD31). P value: <0.0001. h The ratio of PDGFRβ+/CD31+ in Fnip1f/f and FNIP1 MKO GC muscles. n = 7 biologically independent mice per group. i Representative confocal images of immunostainings of CD31 (green) and NG2 (red) co-staining in Fnip1f/f and FNIP1 MKO GC muscles. Scale bar, 100 µm. j Pearson’s correlation of pericyte density (NG2) and Capillary density (CD31). P value: <0.0001. k The ratio of NG2+/CD31+ in Fnip1f/f and FNIP1 MKO GC muscles. n = 9 biologically independent mice per group. l Representative laser Doppler images of 14-week-old Fnip1f/f and FNIP1 MKO mice. m Quantification of hindlimb microcirculation blood perfusion, in perfusion unit (PU). Fnip1f/f, n = 7; FNIP1 MKO, n = 8 biologically independent mice. P value: 0.0004. All data are shown as the mean ± SEM. ***P < 0.001 vs. corresponding Fnip1f/f controls, determined by two-tailed unpaired Student’s t test, except in (g, j) where two-tailed Pearson correlation was used. Source data are provided as a Source Data file.

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