Fig. 1: LSD1 is key mediator in OC and promotes OC progress. | Nature Communications

Fig. 1: LSD1 is key mediator in OC and promotes OC progress.

From: Repression of LSD1 potentiates homologous recombination-proficient ovarian cancer to PARP inhibitors through down-regulation of BRCA1/2 and RAD51

Fig. 1

a–c Representative IHC images (a) and quantification (b, c) of LSD1 protein level in human OC tissues and NATs from TMA (n = 45 paired samples). The quantification analyses were based on staining density scores of IHC (paired two-tailed t test). Scale bar, 400 μm. d, e Representative IHC images (d) and quantification (e) of LSD1 protein level in human FTE (n = 30 samples), HOSE (n = 10 samples) and OC (n = 39 samples) tissues. The quantification analyses were based on staining density scores of IHC (unpaired two-tailed Student’s t test). Scale bar, 50 μm. f Kaplan–Meier plot depicting overall survival of OC patients with tumors expressing high (red) or low (black) levels of LSD1 using TMA (log-rank test). g Cell growth of LSD1 knockdown cells detected by CCK8 assay compared with their control (shCtrl). Data represent mean ± SEM of three biologically independent experiments (two-way ANOVA). h, i Representative images (h) and quantification (i) of colony formation assay for OC cells with LSD1 knockdown. Samples were normalized to shCtrl. Data represent mean ± SEM of three biologically independent experiments (unpaired two-tailed Student’s t test). j–o Tumor volume and tumor weight in shLSD1-expressing and shCtrl-expressing A2780 (j, k), SKOV3 (l, m) and ES2 (n, o) subcutaneous xenografts in nude mice. Data represent mean ± SEM (n = 8 mice per group for A2780 and SKOV3 xenograft models, n = 7 mice per group for ES2 xenograft models; two-way ANOVA for panels j, l, n, and unpaired two-tailed Student’s t test for panels k, m, o). p–s Representative living luminescence images (p, r) and quantification of the luciferase fluorescence signal intensity (q, s) of shLSD1-expressing and shCtrl-expressing A2780 and SKOV3 intraperitoneal xenografts in nude mice. Data represent mean ± SEM (n = 6 mice per group for A2780 xenograft models, n = 7 mice per group for SKOV3 xenograft models; unpaired two-tailed Student’s t test). *p < 0.05; **p < 0.01; ***p < 0.001. Source data and exact p values are provided in the Source Data file.

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