Fig. 5: LSD1 binds BRCA1, BRCA2, and RAD51 gene promoter, regulating these gene transcription dependently of its canonical demethylase function. | Nature Communications

Fig. 5: LSD1 binds BRCA1, BRCA2, and RAD51 gene promoter, regulating these gene transcription dependently of its canonical demethylase function.

From: Repression of LSD1 potentiates homologous recombination-proficient ovarian cancer to PARP inhibitors through down-regulation of BRCA1/2 and RAD51

Fig. 5

a Pie chart showing the genomic distribution of LSD1 peaks based on RefSeq. b Levels of LSD1, H3K9me2, and H3K4me2 bound at the TSS of peaks in ES2 cells, as measured by CUT&Tag-seq analysis. Transcription start site, TSS. c Levels of ATAC bound at the TSS in ES2 cells, as measured by ATAC-seq analysis. d IGV plot showing the distributions of LSD1, H3K9me2, H3K4me2, and ATAC-seq peaks binding in the promoters of BRCA1, BRCA2, and RAD51 in ES2 cells. e ChIP-qPCR analysis showing the enrichment levels of LSD1, H3K9me2 and H3K4me2 at the BRCA1, BRCA2 and RAD51 gene promoter in A2780, SKOV3 and ES2 cells. Data represent the percent of total chromatin input ±SEM of three biologically independent experiments; unpaired two-tailed Student’s t test; ns, not significant). f, g ChIP-qPCR analysis showing the enrichment levels of H3K9me2 and H3K4me2 at the BRCA1, BRCA2 and RAD51 gene promoter. Data represent the percent of total chromatin input ± SEM of three biologically independent experiments (unpaired two-tailed Student’s t test; ns, not significant). h, i RT-qPCR analysis of indicated gene expression. GAPDH was used as the loading control. Data represent mean ± SEM of three biologically independent experiments (unpaired two-tailed Student’s t test). j Western blot analysis of indicated proteins. α-Tubulin was used as the loading control. Numbers below western blot panels represent relative quantification of the respective bands normalized to loading control by densitometry. k Quantification of RAD51 nuclear foci at 4 h after 2 Gy IR treatment in A2780 and ES2 cells. Data represent mean ± SEM of three biologically independent experiments (unpaired two-tailed Student’s t test). l Cell viability in response to olaparib in A2780 and ES2 cells. Data represent mean ± SEM of three biologically independent experiments (two-way ANOVA). ns, not significant, p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. Source data and exact p values are provided as the Source Data file.

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