Fig. 1: Alterations in cellular respiration in sPD patient-derived cells.
From: A reversible state of hypometabolism in a human cellular model of sporadic Parkinson’s disease
a Mitochondrial stress test performed in hiPSCs, b thereof differentiated hNPCs, and c DAns using a Seahorse XFe96 Extracellular Flux Analyzer. Cells were measured in Seahorse XF assay medium supplemented with 25 mM glucose or 5 mM pyruvate (as shown in ref. 17). Injected were (A) Oligomycin (1 μg/ml), (B) carbonyl cyanide p-trifluoro-methoxyphenyl hydrazone (FCCP; 0.5 μM), (C) Rotenone (5 μM)/Antimycin A (2 μM), and (D) 2-deoxyglucose (2-DG; 100 mM). Measurement progression is shown with means ± standard error of the mean (SEM). Boxplots display the median and range from the 25th to 75th percentile. Whiskers extend from the min to max value. Each dot represents one patient. n = 5 Ctrl and 7 sPD patient-derived cell clones, in triplicates. p-values were determined by one-way ANOVA with Sidak’s post hoc test. #p < 0.1; *p < 0.05; **p < 0.01; ***p < 0.001. Source data including p-values are provided as a Source Data file.
