Fig. 3: Abundance of the electron transport chain complexes is not affected in sPD.

a Metabolic KEGG pathways enriched in sPD hNPCs. Single-cell transcriptome data (bulk-like) were previously published¹⁷. FDR-corrected p-values are represented by q-values. b Visualization of the ‘Electron Transport Chain (OXPHOS system in mitochondria)’ pathway based on the single-cell transcriptome data with manual annotations. Color intensities for up- (red) and downregulated (blue) genes are proportional to the fold change. Not significantly altered genes are colored in white. c The abundance of mitochondrial complexes I – V was quantified by western blot with antibodies against the labile subunits NDUFB8 (complex I), SDHB (complex II), UQCRC2 (Complex III), MT-CO2 (Complex IV), and ATP5F1A (Complex V). Expression levels were normalized to ACTB or α-Tubulin levels. Western blots are exemplarily shown for some hNPC clones. Quantifications of protein levels are shown for d hNPCs and e DAns. n = 5 Ctrl and 7 sPD patient-derived cell clones, in triplicates. Boxplots display the median and range from the 25th to 75th percentile. Whiskers extend from the min to max value. Each dot represents one patient. p-values were determined by one-sided hypergeometric tests (a), two-sided t-test (d) (complex I: p = 0.69; complex II: p = 0.86; complex III: p = 0.89; complex IV: p = 0.66; complex V: p = 0.83), e (complex I: p = 0.35; complex II: p = 0.99; complex III: p = 0.62; complex IV: p = 0.50; complex V: p = 0.90). *p < 0.05; **p < 0.01; ***p < 0.001. Source data are provided as a Source Data file.