Fig. 8: Imiquimod induces the release of mast cell tryptase from human mast cells.
From: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
a Representative micrographs (top panels, n = 3) and color deconvolution of isotype control (left panels) and TLR7 (right panels) immunostaining of HMC-1 human mast cells. Scale bars, 50 µm. b Representative micrographs (top panels, n = 3) and color deconvolution (bottom panels) of isotype control (left panel) and mast cell tryptase (right panels) immunostaining of HMC-1 cells incubated with media or imiquimod (5, 10 or 100 ng) for 1 h, Scale bars, 50 µm. Quantification of mast cell tryptase in cells (10 random fields per sample, n = 3 per group) normalized to c number of cells or d area of hematoxylin of HMC-1 cells incubated with media or imiquimod (5, 10 or 100 ng) for 1 h. e Quantification of mast cell tryptase activity (n = 6 per group) in terms of p-nitroaniline levels in culture supernatants from HMC-1 cells incubated with media or imiquimod (5, 10, or 100 ng) for 1 h. Throughout, data are presented as means ± s.e.m. Statistical analysis was performed using one-way ANOVA with Bonferroni’s multiple comparison test. Source data are provided as a Source Data file.
