Fig. 7: The PENK-A–H₂O₂ pathway regulates kidney regeneration through tcf21.

a Confocal images showing adult Tg(lhx1a:DsRed) kidneys at 5 dpi afer administration (at 2 and 4 dpi) of tcf21 vivo-MO, or Con vivo-MO following AKI (n = 5 biological replications per group). b Quantitation of individual RPCs (iRPCs, arrowhead) and RPCAs in a. c, d RT-PCR (n = 3) (c) and WISH (d) analyses of lhx1a in 7 dpi WT and penka^−/− kidneys with administration (at 2, 4, and 6 dpi) of tcf21 vivo-MO (n = 5 in WT group, n = 8 in penka^−/− group) or Con vivo-MO ((n = 5 in WT group, n = 4 in penka^−/− group) following AKI. e The lhx1a⁺ RPCAs per kidney were quantified for each condition in d. f Confocal images showing 5 dpi Tg(lhx1a:DsRed;hsp70l:tcf21) kidneys with HS (at 2 and 4 dpi) or Un-HS after administration (at 2 and 4 dpi) of Met-ENK (n = 9 in Un-HS group, n = 5 in HS group), CPI-455 (n = 9 in Un-HS group, n = 6 in HS group), or DMSO (n = 8) following AKI. g Quantitation of individual RPCs (iRPCs, arrowheads) and RPCAs in (f). h RT-PCR analysis of tcf21 and lhx1a in 7 dpi WT and Tg(hsp70l:tcf21) kidneys with HS (at 2, 4, and 6 dpi) or Un-HS after administration (at 2, 4, and 6 dpi) of Met-ENK, CPI-455 or DMSO following AKI (n = 3). i WISH analysis of lhx1a in 7 dpi WT and Tg(hsp70l:tcf21) kidneys with HS (at 2 and 4 dpi) or Un-HS after administration (at 2, 4, and 6 dpi) of Met-ENK (n = 5), CPI-455 (n = 5) or DMSO (n = 5 in Un-HS group, n = 4 in HS group) following AKI. j Quantitation of lhx1a⁺ RPCAs per kidney was performed for each condition in (i). Data in (b), (e), (g), and (j) were analyzed by two-sided t-test and are presented as mean values  ±  SD. Scale bars in (a) and (f), 50 μm; (d) and (i), 600 μm. Source data are provided as a Source data file.