Fig. 3: SERS spectra of single proteins. | Nature Communications

Fig. 3: SERS spectra of single proteins.

From: Accessible hotspots for single-protein SERS in DNA-origami assembled gold nanorod dimers with tip-to-tip alignment

Fig. 3

a DFM image of the corresponding dimer nanoantenna and sketch illustrating the nanogap filled with one biotin molecule, with example SERS spectra of only biotin (intensity in counts: cts). b Sketch illustrating the process of streptavidin entering the hotspot and binding to biotin, with SERS spectra obtained after adding streptavidin. The first spectrum in the stack was obtained shortly before streptavidin was captured by the antenna. Additional peaks corresponding to streptavidin are observed after the protein entered the nanogap (three bottom spectra). Raman peaks indicative of biotin and streptavidin are highlighted with green and blue bars respectively (see Table 1 for peak assignment). c DFM image of the corresponding dimer nanoantenna and sketch illustrating the nanogap filled with one aptamer, with SERS spectrum obtained from a dimer nanoantenna with HD22 before thrombin attachment. d Sketch illustrating the thrombin capturing process, with combined HD22/thrombin SERS spectra. All SERS spectra were obtained with 0.5 s integration times. Raman peaks indicative of HD22 or thrombin, are highlighted with grey and red bars respectively (see Table 1 for peak assignment). Source data are provided as a Source Data file.

Back to article page