Fig. 1: The point mutation H527P constrains VZV gB in a prefusion conformation.
From: Targeted mutagenesis of the herpesvirus fusogen central helix captures transition states
a A diagram of the linear structure for VZV gB highlighting the ___location of the DIII central helix. The signal sequence is depicted by the crossed white box. Colored regions (residues 115–736) correspond to domains in the cryo-EM structure; DI (cyan), DII (green), DIII (yellow), DIV (orange), DV (red) and linker regions (hot pink). Amino acids 526EHV528 (bold) were targeted for mutagenesis. b and c Trimer and monomer models for VZV gB prefusion (b) by SWISS Model20 and postfusion (c) from the 2.8 Å cryo-EM structure3. The inset shows the 526EHV528 site in the DIII central helix. d Single plane views of representative tomographic reconstructions (see Supplementary Table 1) from cryo-ET of EVs produced by BHK-21 cells transfected with gB[H527P] or WT gB expression vectors. Upper and lower panels are different planes from the same tomogram to show side and top views of gB[H527P] or WT gB expressed on EVs. Boxes with dashed lines are magnified areas shown in the insets. Scale bars shown are for each panel and inset.
